|
|
Application of KPM on Graphene/Si Surface Modified by FIB method
Konečný, Martin ; Rezek, Bohuslav (referee) ; Bartošík, Miroslav (advisor)
This diploma thesis is focused on the application of Kelvin probe microscopy on graphene fabricated by the chemical vapour deposition. The theoretical part of the thesis deals with basic principles of Kelvin force microscopy and focus ion beam. Further, basic properties of graphene and its possible fabrication methods are discussed. The experimental part is focused on the surface potential measurements on graphene membranes fabricated on the substrate modified by focus ion beam. Finally, atomic force microscope lithography was used for nanopatterning of graphene sheets.
|
|
|
Prediction of the Effect of Mutation on Protein Solubility
Velecký, Jan ; Martínek, Tomáš (referee) ; Hon, Jiří (advisor)
The goal of the thesis is to create a predictor of the effect of a mutation on protein solubility given its initial 3D structure. Protein solubility prediction is a bioinformatics problem which is still considered unsolved. Especially a prediction using a 3D structure has not gained much attention yet. A relevant knowledge about proteins, protein solubility and existing predictors is included in the text. The principle of the designed predictor is inspired by the Surface Patches article and therefore it also aims to validate the results achieved by its authors. The designed tool uses changes of positive regions of the electric potential above the protein's surface to make a prediction. The tool has been successfully implemented and series of computationally expensive experiments have been performed. It was shown that the electric potential, hence the predictor itself too, can be successfully used just for a limited set of proteins. On top of that, the method used in the article correlates with a much simpler variable - the protein's net charge.
|
|
|
Study of selective hydrogenation using KPFM
Zeman, Filip ; Konečný, Martin (referee) ; Bartošík, Miroslav (advisor)
The bachelor thesis deals with the possibilities of selective hydrogenation of graphene and their analysis. The theoretical part deals with the properties of carbon materials and different hydrogenation methods. Furthermore, it thoroughly describes the raster probe microscopy AFM and KPFM. The last part of the theory discusses a suitable method to analyze hydrogenated graphene, namely Raman spectroscopy. The results that have already been observed in previous research are discussed. In the experimental part, three methods for producing hydrogenated graphene samples are described, which are measured using KPFM and Raman spectroscopy. Finally, their results are compared.
|
|
|
Prediction of the Effect of Mutation on Protein Solubility
Velecký, Jan ; Martínek, Tomáš (referee) ; Hon, Jiří (advisor)
The goal of the thesis is to create a predictor of the effect of a mutation on protein solubility given its initial 3D structure. Protein solubility prediction is a bioinformatics problem which is still considered unsolved. Especially a prediction using a 3D structure has not gained much attention yet. A relevant knowledge about proteins, protein solubility and existing predictors is included in the text. The principle of the designed predictor is inspired by the Surface Patches article and therefore it also aims to validate the results achieved by its authors. The designed tool uses changes of positive regions of the electric potential above the protein's surface to make a prediction. The tool has been successfully implemented and series of computationally expensive experiments have been performed. It was shown that the electric potential, hence the predictor itself too, can be successfully used just for a limited set of proteins. On top of that, the method used in the article correlates with a much simpler variable - the protein's net charge.
|
|
|
Application of KPM on Graphene/Si Surface Modified by FIB method
Konečný, Martin ; Rezek, Bohuslav (referee) ; Bartošík, Miroslav (advisor)
This diploma thesis is focused on the application of Kelvin probe microscopy on graphene fabricated by the chemical vapour deposition. The theoretical part of the thesis deals with basic principles of Kelvin force microscopy and focus ion beam. Further, basic properties of graphene and its possible fabrication methods are discussed. The experimental part is focused on the surface potential measurements on graphene membranes fabricated on the substrate modified by focus ion beam. Finally, atomic force microscope lithography was used for nanopatterning of graphene sheets.
|
guest ::
